SHMT2 (NM_005412) Human Recombinant Protein

• Background:

  This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

• Synonyms:

  GLYA; HEL-S-51e; NEDCASB; SHMT

• Gene ID:

  6472

• UniProt:

  P34897, V9HW06, Q5BJF5

• Accession Number mRNA:

  NM_005412

• Chromosomal Location:

  12q13.3

• Expression System:

  E. coli

• Tag:

  N-His

• Related Pathways:

  Cyanoamino acid metabolism, Glycine, serine and threonine metabolism, Metabolic pathways, Methane metabolism, One carbon pool by folate

• Field of Research:

  Cyanoamino acid metabolism, Glycine, serine and threonine metabolism, Metabolic pathways, Methane metabolism, One carbon pool by folate

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl

• Molecular Weight:

  56

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  SHMT2

• Species:

  Human

• Protein ID:

  NP_005403

• Overview:

  Recombinant protein of human serine hydroxymethyltransferase 2 (mitochondrial) (SHMT2), nuclear gene encoding mitochondrial protein, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/6472

• Uniprot URL:

  https://www.uniprot.org/uniprot/P34897

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_005412

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_005403