KARS Recombinant Protein

• Gene Name :

  Lysyl-tRNA synthetase 1

• Gene Aliases :

  CMTRIB; DEAPLE; DFNB89; KARS; KARS2; KRS; LEPID; lysine tRNA ligase; lysine--tRNA ligase; lysRS; Lysyl-tRNA synthetase.

• Gene ID :

  3735

• Accession Number :

  NP_001123561.1

• Reactivity :

  Homo sapiens|Human

• Target :

  Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:9278442, PubMed:18029264, PubMed:18272479) . When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages (PubMed:15851690) . Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity (PubMed:5338216, PubMed:14975237, PubMed:19524539, PubMed:23159739) .

• Conjugation :

  Unconjugated

• Type :

  Protein

• Source :

  E.coli

• Applications :

  Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration :

  200 ug/mL (prior to lyoph)

• Format :

  Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

• Reconstitution :

  2°C to 8°C|-80°C

• Molecular Weight :

  30kDa

• Protein Length :

  Recombinant

• NCBI Gene Symbol :

  KARS1

• Host or Source :

  E.coli

• Protein Name :

  Lysine--tRNA ligase

• Gene Name URL :

  KARS1

• CAS Number :

  9000-83-3

• Nucleotide Accession Number :

  NM_001130089.1