KARS ELISA Kit (Human)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Lysyl tRNA Synthetase (KARS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Lysyl tRNA Synthetase (KARS) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Lysyl-tRNA synthetase 1

• Gene Aliases:

  CMTRIB; DEAPLE; DFNB89; KARS; KARS2; KRS; LEPID; lysine tRNA ligase; lysine--tRNA ligase; lysRS; Lysyl-tRNA synthetase.

• Gene ID:

  3735

• Accession Number:

  NP_001123561

• Reactivity:

  Homo sapiens|Human

• Target:

  Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:9278442, PubMed:18029264, PubMed:18272479) . When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages (PubMed:15851690) . Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity (PubMed:5338216, PubMed:14975237, PubMed:19524539, PubMed:23159739) .

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysyl tRNA Synthetase (KARS) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysyl tRNA Synthetase (KARS) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysyl tRNA Synthetase (KARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lysyl tRNA Synthetase (KARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma, tissue homogenates and other biological fluids

• Detection Range:

  0.312-20ng/mL

• Recovery:

  Matrix Recovery Range (%) Average (%) serum (n=5) 91-105 99 EDTA plasma (n=5) 80-91 86 heparin plasma (n=5) 83-101 93 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 91-105 99 serum (n=5) 91-105 99 EDTA plasma (n=5) 80-91 86 EDTA plasma (n=5) 80-91 86 heparin plasma (n=5) 83-101 93 heparin plasma (n=5) 83-101 93

• Sensitivity:

  < 0.117 ng/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Anti-KARS Microplate 96 Wells (12 x 8 Well Strips) KARS Lyophilized Standard 2 x 20 ng 100X Biotinylated KARS Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-KARS Microplate 96 Wells (12 x 8 Well Strips) Anti-KARS Microplate 96 Wells (12 x 8 Well Strips) KARS Lyophilized Standard 2 x 20 ng KARS Lyophilized Standard 2 x 20 ng 100X Biotinylated KARS Detector Antibody 120 uL 100X Biotinylated KARS Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• Notes:

  Formerly GWB-KBBDN4

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Lysyl tRNA Synthetase (KARS) . No significant cross-reactivity or interference between Lysyl tRNA Synthetase (KARS) and analogues was observed.

• Protein Length:

  597

• NCBI Gene Symbol:

  KARS1

• Protein Name:

  Lysine--tRNA ligase

• Gene Name URL:

  KARS1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001130089.1