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KARS ELISA Kit (Mouse)

CAT: 0247-OKCD02668-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02668-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Lysyl tRNA Synthetase (KARS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Lysyl tRNA Synthetase (KARS) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Lysyl-tRNA synthetase
Gene Aliases
AA589550; AL024334; AL033315; AL033367; D8Ertd698; D8Ertd698e; D8Wsu108; D8Wsu108e; Kars1; L; lysine--tRNA ligase; LysRS; Lysyl-tRNA synthetase; mKIAA0070.
Gene ID
85305
Accession Number
NP_444322.1
Reactivity
Mouse|Mus musculus
Target
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysyl tRNA Synthetase (KARS) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysyl tRNA Synthetase (KARS) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysyl tRNA Synthetase (KARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lysyl tRNA Synthetase (KARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 79-96 78 EDTA Plasma (n=5) 97-105 101 Heparin Plasma (n=5) 81-92 84 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 79-96 78 Serum (n=5) 79-96 78 EDTA Plasma (n=5) 97-105 101 EDTA Plasma (n=5) 97-105 101 Heparin Plasma (n=5) 81-92 84 Heparin Plasma (n=5) 81-92 84
Sensitivity
13.3 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-KARS Microplate 96 Wells (12 x 8 Well strips) KARS Lyophilized Standard 2 100X Biotinylated KARS Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-KARS Microplate 96 Wells (12 x 8 Well strips) Anti-KARS Microplate 96 Wells (12 x 8 Well strips) KARS Lyophilized Standard 2 KARS Lyophilized Standard 2 100X Biotinylated KARS Detector Antibody 1 x 120 uL 100X Biotinylated KARS Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Lysyl tRNA Synthetase (KARS) . No significant cross-reactivity or interference between Lysyl tRNA Synthetase (KARS) and analogues was observed.
Protein Length
595
NCBI Gene Symbol
Kars
Protein Name
Lysine--tRNA ligase
Gene Name URL
Kars
Nucleotide Accession Number
NM_053092.3

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