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KAE1 Recombinant Protein

CAT: 0247-OPCA03107-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0247-OPCA03107-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9000-83-3
Gene Name
TRNA N6-adenosine threonylcarbamoyltransferase
Gene Aliases
Kinase-associated endopeptidase 1; N6-L-threonylcarbamoyladenine synthase; t (6) A37 threonylcarbamoyladenosine biosynthesis protein KAE1; tRNA N6-adenosine threonylcarbamoyltransferase; tRNA threonylcarbamoyladenosine biosynthesis protein KAE1; YKR038C.
Gene ID
853910
Accession Number
NP_012964.2
Reactivity
Saccharomyces cerevisiae
Target
Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t (6) A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. KAE1 likely plays a direct catalytic role in this reaction, but requires other protein (s) of the complex to fulfill this activity. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators.
Type
Protein
Source
Yeast
Sequence
MVNLNTIPPKNGRDYYIALGLEGSANKLGVGIVKHPLLPKHANSDLSYDCEAEMLSNIRDTYVTPPGEGFLPRDTARHHRNWCIRLIKQALAEADIKSPTLDIDVICFTKGPGMGAPLHSVVIAARTCSLLWDVPLVGVNHCIGHIEMGREITKAQNPVVLYVSGGNTQVIAYSEKRYRIFGETLDIAIGNCLDRFARTLKIPNEPSPGYNIEQLAKKAPHKENLVELPYTVKGMDLSMSGILASIDLLAKDLFKGNKKNKILFDKTTGEQKVTVEDLCYSLQENLFAMLVEITERAMAHVNSNQVLIVGGVGCNVRLQEMMAQMCKDRANGQVHATDNRFCIDNGVMIAQAGLLEYRMGGIVKDFSETVVTQKFRTDEVYAAWRD
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Format
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
-20°C or -80°C
Molecular Weight
44.7 kDa
Protein Length
Recombinant
NCBI Gene Symbol
KAE1
Host or Source
Yeast
Protein Name
TRNA N6-adenosine threonylcarbamoyltransferase
Gene Name URL
KAE1
Nucleotide Accession Number
NM_001179828.1

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MBS1384129-01Recombinant Kluyveromyces lactis Probable tRNA threonylcarbamoyladenosine biosynthesis protein KAE1 (KAE1)
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