ON Recombinant Protein

• Gene Name:

  Secreted protein acidic and cysteine rich

• Gene Aliases:

  Basement-membrane protein 40; BM-40; ON; osteonectin; secreted acidic cysteine rich glycoprotein; Secreted acidic cystein-rich glycoprotein (osteonectin) ; secreted protein acidic and rich in cysteine; secreted protein, acidic, cysteine-rich (osteonectin) ; SPARC.

• Gene ID:

  24791

• Accession Number:

  NP_036788.1

• Reactivity:

  Rat|Rattus norvegicus

• Target:

  Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca (2+) with a low affinity and an EF-hand loop that binds a Ca (2+) ion with a high affinity.

• Conjugation:

  Unconjugated

• Type:

  Protein

• Source:

  E.coli

• Applications:

  Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  200 ug/mL (prior to lyoph)

• Format:

  Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

• Reconstitution:

  2°C to 8°C|-80°C

• Molecular Weight:

  33kDa

• Protein Length:

  Recombinant

• NCBI Gene Symbol:

  Sparc

• Host or Source:

  E.coli

• Protein Name:

  SPARC

• Gene Name URL:

  Sparc

• CAS Number:

  9000-83-3

• Nucleotide Accession Number:

  NM_012656.1