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S100A15A ELISA Kit (Mouse)

CAT: 0247-OKWB00258-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKWB00258-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Name
S100 calcium binding protein A7A
Gene Aliases
AY465109; Gm1020; protein S100-A15A; Protein S100-A7A; S100 calcium binding protein A15; S100 calcium binding protein A7-like 1; S100 calcium-binding protein A15A; S100 calcium-binding protein A7A; S100A; S100a1; S100a15; S100a15a; S100a17l1; S100A7f; S100A7L1.
Gene ID
381493
Accession Number
NP_955454
Reactivity
Mouse|Mus musculus
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric, OD 450 nm
Assay Principle
Aviva Systems Biology S100a15a ELISA Kit (Mouse) (OKWB00258) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for S100a15a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for S100a15a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample S100a15a captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
0.234-15ng/ml
Recovery
Sample Type Range Mean Serum (n=5) 85-99 90 EDTA Plasma (n=5) 91-104 98 Heparin Plasma (n=5) 88-105 95 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 85-99 90 Serum (n=5) 85-99 90 EDTA Plasma (n=5) 91-104 98 EDTA Plasma (n=5) 91-104 98 Heparin Plasma (n=5) 88-105 95 Heparin Plasma (n=5) 88-105 95
Sensitivity
0.141 ng/mL
Reconstitution
Store as indicated in product manual.
Components
Component Amount S100a15a Microplate 96 Wells (12 x 8 Well strips) S100a15a Lyophilized Standard 2 x 15 ng 100X S100a15a Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount S100a15a Microplate 96 Wells (12 x 8 Well strips) S100a15a Microplate 96 Wells (12 x 8 Well strips) S100a15a Lyophilized Standard 2 x 15 ng S100a15a Lyophilized Standard 2 x 15 ng 100X S100a15a Biotinylated Detector Anitbody 1 x 120 uL 100X S100a15a Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL
NCBI Gene Symbol
S100a7a
Protein Name
Protein S100-A15A
Gene Name URL
S100a7a
Nucleotide Accession Number
NM_199422

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