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S100a13 ELISA Kit (Mouse)

CAT: 0247-OKBB01094-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB01094-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 154 408 31 143 400 Standard deviation 1.38 8 29.78 1.82 8.29 31.6 CV (%) 4.2 5.2 7.3 5.9 5.8 7.9 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 154 408 31 143 400 Mean (pg/ml) 33 154 408 31 143 400 Standard deviation 1.38 8 29.78 1.82 8.29 31.6 Standard deviation 1.38 8 29.78 1.82 8.29 31.6 CV (%) 4.2 5.2 7.3 5.9 5.8 7.9 CV (%) 4.2 5.2 7.3 5.9 5.8 7.9
CAS Number
7732-18-5
Gene Name
S100 calcium binding protein A13
Gene Aliases
Protein S100 A13, Protein S100-A13, S100 calcium binding protein A13, S100 calcium-binding protein A13, S100a13, S10AD_HUMAN, Q99584
Gene ID
20196
Accession Number
NP_033139.2
Reactivity
Mouse
Immunogen
Expression system for standard: E.coli; Immunogen sequence: M1-K98
Target
S100 calcium-binding protein A13 (S100A13) is a protein that in humans is encoded by the S100A13 gene. The characterization of a human gene, S100A13 encodes a calcium-binding protein belonging to the S100 protein family. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is widely expressed in various types of tissues with a high expression level in thyroid gland. In smooth muscle cells, this protein co-expresses with other family members in the nucleus and in stress fibers, suggesting diverse functions in signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse S100A13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for S100A13 has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: M1-K98) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for S100A13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse S100A13 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse S100A13
NCBI Gene Symbol
S100A13
Protein Name
Protein S100-A13
Gene Name URL
S100A13
Nucleotide Accession Number
NM_009113.4

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