Products for Research Use Only
RegisterLogin

ZGLP1 ELISA Kit (Mouse)

CAT: 0247-OKWB00253-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKWB00253-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Name
Zinc finger, GATA-like protein 1
Gene Aliases
GATA-like 1; GATA-like protein 1; GATA-type zinc finger protein 1; Gl; Glp1; GLP-1.
Gene ID
100009600
Accession Number
NP_001096638
Reactivity
Mouse|Mus musculus
Target
Transcriptional repressor that plays a central role in somatic cells of the gonad and is required for germ cell development. Able to repress GATA transcription factor function.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric, OD 450 nm
Assay Principle
Aviva Systems Biology Zglp1 ELISA Kit (Mouse) (OKWB00253) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Zglp1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Zglp1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Zglp1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
15.625-1000pg/ml
Recovery
Sample Type Range Mean Serum (n=5) 86-103 98 EDTA Plasma (n=5) 88-103 94 Heparin Plasma (n=5) 87-105 98 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 86-103 98 Serum (n=5) 86-103 98 EDTA Plasma (n=5) 88-103 94 EDTA Plasma (n=5) 88-103 94 Heparin Plasma (n=5) 87-105 98 Heparin Plasma (n=5) 87-105 98
Sensitivity
9.4 pg/mL
Reconstitution
Store as indicated in product manual.
Components
Component Amount Zglp1 Microplate 96 Wells (12 x 8 Well strips) Zglp1 Lyophilized Standard 2 x 1 ng 100X Zglp1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount Zglp1 Microplate 96 Wells (12 x 8 Well strips) Zglp1 Microplate 96 Wells (12 x 8 Well strips) Zglp1 Lyophilized Standard 2 x 1 ng Zglp1 Lyophilized Standard 2 x 1 ng 100X Zglp1 Biotinylated Detector Anitbody 1 x 120 uL 100X Zglp1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL
NCBI Gene Symbol
Zglp1
Protein Name
GATA-type zinc finger protein 1
Gene Name URL
Zglp1
Nucleotide Accession Number
NM_001103168

Chemical Information

Water

Water is an oxygen hydride consisting of an oxygen atom that is covalently bonded to two hydrogen atoms. It has a role as an amphiprotic solvent, a mouse metabolite, a Saccharomyces cerevisiae metabolite, a member of greenhouse gas, an Escherichia coli metabolite and a human metabolite. It is an oxygen hydride, a mononuclear parent hydride and an inorganic hydroxy compound. It is a conjugate base of an oxonium. It is a conjugate acid of a hydroxide.

CAS Number7732-18-5
PubChem CID962
IUPAC Nameoxidane
Molecular FormulaH2O
Molecular Weight18.015
XLogP-0.5
Topological Polar Surface Area1
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Heavy Atom Count1
Formal Charge0
Complexity0
SMILES
O
InChI
InChI=1S/H2O/h1H2
InChIKey
XLYOFNOQVPJJNP-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Water
CAS: 7732-18-5
CID: 962
Formula: H2O
MW: 18.015
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight18.015
XLogP3-0.5
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Exact Mass18.010564683
Monoisotopic Mass18.010564683
Topological Polar Surface Area1 Ų
Heavy Atom Count1
Formal Charge0
Complexity0
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

Related Products

CatalogName
OCOA18209-20UGZGLP1 Protein Lysate
OKCD06004-96WKIT ELISA Kit (Mouse)
OKEH00204-96WKIT ELISA Kit (Mouse)
OKCD06000-96WKITL ELISA Kit (Mouse)
OKCD06004KIT ELISA Kit (Mouse) (OKCD06004)
OKBB01105Kit ELISA Kit (Mouse) (OKBB01105)
OKAN04824KITL ELISA Kit (Mouse) (OKAN04824)
OKAN04825KITL ELISA Kit (Mouse) (OKAN04825)
OKCD06000KITL ELISA Kit (Mouse) (OKCD06000)
OKCD08592-96WPLIN4 ELISA Kit (Mouse)

Alternative Products

CatalogName
MBS2890901-01Mouse ZGLP1/GATA-Type Zinc Finger Protein 1 ELISA Kit
ELI-40699mMouse GATA-type zinc finger protein 1, Zglp1 ELISA KIT
MBS8203955-01ZGLP1 siRNA (Mouse)
51239054ZGLP1 Adenovirus (Mouse)
abx940471-01Mouse ZGLP1 siRNA
E103411-01Human ZGLP1 ELISA Kit
abx984272-01Mouse ZGLP1 shRNA Plasmid
RP188039ZGLP1 Recombinant Protein (Mouse)
GTR15210641Lentiviral mouse Zglp1 shRNA (UAS) - Lentiviral mouse Zglp1 shRNA (UAS) (25)
GTR15210642Lentiviral mouse Zglp1 shRNA (UAS) - Lentiviral mouse Zglp1 shRNA (UAS) (100)

Popular Products