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HSPA9 ELISA Kit (Rat)

CAT: 0247-OKEI00784-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEI00784-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Aliases
75 kDa glucose-regulated protein, Grp75, GRP-75, Heat shock 70 kDa protein 9, Hspa9a, Mortalin, mtHSP70, PBP74, Peptide-binding protein 74, Stress-70 protein, mitochondrial
Reactivity
Rat
Target
Chaperone protein which is implicated in the control of cell proliferation and cellular aging. Plays a role in the erythropoiesis process.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Hspa9 ELISA Kit (Rat) (OKEI00784) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hspa9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hspa9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Hspa9
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Biological Fluids
Detection Range
0.313 - 20 ng/mL
Sensitivity
0.188 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
HSPA9
Protein Name
Stress-70 protein, mitochondrial
Gene Name URL
HSPA9

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