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HSPA2 ELISA Kit (Rat)

CAT: 0247-OKEH05928-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH05928-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Heat shock protein family A (Hsp70) member 2
Gene Aliases
Heat shock 70kDa protein 2; heat shock protein 2; heat shock protein 70.2; heat shock protein alpha 2; heat shock protein family A member 2; heat shock-related 70 kDa protein 2; Hspt70; HST; Hst70; Testis-specific heat shock protein-related; testis-specific heat shock protein-related gene hst70.
Gene ID
60460
Accession Number
NP_068635.1
Reactivity
Rat, Rattus norvegicus
Target
Heat shock-related 70 kDa protein 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Hspa2 ELISA Kit (Rat) (OKEH05928) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hspa2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Hspa2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hspa2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 104%
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Heat shock-related 70 kDa protein 2
Protein Length
633
NCBI Gene Symbol
Hspa2
Protein Name
P14659
Gene Name URL
Hspa2
Nucleotide Accession Number
NM_021863.3

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