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CCNE1 ELISA Kit (Mouse)

CAT: 0247-OKEI00582-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEI00582-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Aliases
CycE1, AW538188
Gene ID
12447
Accession Number
NP_031659
Reactivity
Mouse
Target
Essential for the control of the cell cycle at the G1/S (start) transition.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Ccne1 ELISA Kit (Mouse) (OKEI00582) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ccne1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ccne1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Ccne1
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Biological Fluids
Detection Range
0.078 - 5 ng/mL
Sensitivity
46.9 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
CCNE1
Protein Name
G1/S-specific cyclin-E1
Gene Name URL
CCNE1
Nucleotide Accession Number
NM_007633

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