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CCNE1 ELISA Kit (Human)

CAT: 0247-OKCA01250-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA01250-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Cyclin E1
Gene Aliases
CCNE, pCCNE1
Gene ID
898
Accession Number
NP_001229.1
Reactivity
Human
Target
Essential for the control of the cell cycle at the G1/S (start) transition.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology CCNE1 ELISA Kit (Human) (OKCA01250) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CCNE1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCNE1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CCNE1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Lysates
Detection Range
25 - 1,600 pg/mL
Recovery
Sample Mean Recovery (%) Range (%) Serum (n=5) 93 89-97 EDTA Plasma (n=4) 88 84-92 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 93 89-97 Serum (n=5) 93 89-97 EDTA Plasma (n=4) 88 84-92 EDTA Plasma (n=4) 88 84-92
Sensitivity
6.25 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
CCNE1
Protein Name
G1/S-specific cyclin-E1
Gene Name URL
CCNE1
Nucleotide Accession Number
NM_001238.3

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