Products for Research Use Only
RegisterLogin

ATF4 ELISA Kit (Mouse)

CAT: 0247-OKEH08745-96WSize: 96 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKEH08745-96WSize:96 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Activating transcription factor 4
Gene Aliases
Activating transcription factor 4; Atf-; Atf-4; C/AT; C/ATF; c/EBP-related ATF; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB; CREB2; CREB-2; cyclic AMP-dependent transcription factor ATF-4; cyclic AMP-responsive element-binding protein 2; TAXREB; TAXREB67; taxREB67 homolog; tax-responsive enhancer element-binding protein 67 homolog.
Gene ID
11911
Accession Number
NP_001274109.1
Reactivity
Mouse, Mus musculus
Target
Cyclic AMP-dependent transcription factor ATF-4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ATF4 ELISA Kit (Mouse) (OKEH08745) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATF4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATF4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATF4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
0.156-10 ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 114% (n = 20)
Sensitivity
0.079 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Cyclic AMP-dependent transcription factor ATF-4
NCBI Gene Symbol
Atf4
Protein Name
Q06507
Gene Name URL
Atf4
Nucleotide Accession Number
NM_001287180.1

Related Products

CatalogName
OKDD00138-96WATF4 ELISA Kit (Human)
OKCD07373-96WATF4 ELISA Kit (Human)
OKCD01199-96WATF4 ELISA Kit (Rat)
OKDD00138ATF4 ELISA Kit (Human) (OKDD00138)
OKAN06020ATF4 ELISA Kit (Human) (OKAN06020)
OKCD07373ATF4 ELISA Kit (Human) (OKCD07373)
OKCD01199ATF4 ELISA Kit (Rat) (OKCD01199)
OAAN00014-50ULATF4 Antibody
OAAN00014-100ULATF4 Antibody
OAAF01242-100UGATF4 Antibody

Alternative Products

CatalogName
E042607Mouse ATF4 ELISA Kit
MBS8820621-01Mouse ATF4 (Activating Transcription Factor 4) ELISA Kit
BG-MUS10167Mouse Activating Transcription Factor 4 ATF4 ELISA Kit
JL39738-01Mouse Activating Transcription Factor 4 (ATF4) ELISA Kit
EK5F41-01Mouse Activating Transcription Factor 4 (ATF4) ELISA Kit-Sandwich
RK12715Mouse Cyclic AMP-dependent transcription factor ATF-4 (ATF4) ELISA Kit
MBS2891125-01Mouse ATF4/Cyclic AMP-Dependent Transcription Factor ATF-4 ELISA Kit
C6786Cyclic AMP-dependent Transcription Factor ATF-4 (ATF4) Mouse ELISA Kit
GTR10401951Mouse Cyclic AMP dependent transcription factor ATF 4 (ATF4) ELISA kit
YP-W60394-01Chicken ATF4 (ATF4) ELISA Kit

Popular Products