Products for Research Use Only
RegisterLogin

ATF4 ELISA Kit (Human)

CAT: 0247-OKDD00138-96WSize: 96 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKDD00138-96WSize:96 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level ATF4 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level ATF4 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV Inter-Assay: CV
CAS Number
7732-18-5
Gene Name
Activating transcription factor 4
Gene Aliases
Activating transcription factor 4; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB2; CREB-2; cyclic AMP-dependent transcription factor ATF-4; cyclic AMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; TAXREB67; tax-responsive enhancer element B67; tax-responsive enhancer element-binding protein 67; TXREB.
Gene ID
468
Accession Number
NP_001666.2
Reactivity
Homo sapiens|Human
Target
Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production (By similarity) . It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. Regulates the induction of DDIT3/CHOP and asparagine synthetase (ASNS) in response to endoplasmic reticulum (ER) stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements (CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes. During ER stress response, activates the transcription of NLRP1, possibly in concert with other factors (PubMed:26086088) .
Type
ELISA Kit
Applications
Enzyme-linked immunosorbent assay
Assay Principle
Aviva Systems Biology ATF4 ELISA Kit (Human) (OKDD00138) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for ATF4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATF4 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ATF4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell lysates
Detection Range
0.156-10ng/mL
Sensitivity
< 0.057 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
39 kDa
Notes
Formerly GWB-KBBGB6
Specificity
This assay has high sensitivity and excellent specificity for detection of ATF4. No significant cross-reactivity or interference between ATF4 and analogues was observed.
Protein Length
351
NCBI Gene Symbol
ATF4
Protein Name
Cyclic AMP-dependent transcription factor ATF-4
Gene Name URL
ATF4
Nucleotide Accession Number
NM_001675.4

Related Products

CatalogName
OKEH08745-96WATF4 ELISA Kit (Mouse)
OKCD01199-96WATF4 ELISA Kit (Rat)
OKEH08745ATF4 ELISA Kit (Mouse) (OKEH08745)
OKCD01199ATF4 ELISA Kit (Rat) (OKCD01199)
OAAN00014-50ULATF4 Antibody
OAAN00014-100ULATF4 Antibody
OAAF01242-100UGATF4 Antibody
OAAN00014-200ULATF4 Antibody
OAAJ06482-100ULATF4 Antibody
OASE00295-100UGATF4 Antibody

Alternative Products

CatalogName
EHA0541Human ATF4 ELISA Kit
NBP2-69883Human ATF4 ELISA Kit (Colorimetric)
GTR18828213-01Human ATF4 ELISA Kit (Ready to Use)
JOT-EK7443Hu-01Human Activating Transcription Factor 4,ATF4 ELISA KIT
MBS452643-01Human Activating Transcription Factor 4 (ATF4) ELISA Kit
MBS1608927-01Human Activating Transcription Factor 4, ATF4 ELISA Kit
BG-HUM10167Human Activating Transcription Factor 4 ATF4 ELISA Kit
RK00955Human Activating Transcription Factor 4 ELISA Kit (ATF4)
EKE60365Human ATF4 (Activating Transcription Factor 4) ELISA Kit
B2435Activating Transcription Factor 4 (ATF4) Human ELISA Kit

Popular Products