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ITPRID2 ELISA Kit (Mouse)

CAT: 0247-OKEH08727-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH08727-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
ITPR interacting domain containing 2
Gene Aliases
5730488C15Rik; CS; CS-; CS1; CS-1; ITPR-interacting domain-containing protein 2; K; ki-ras-induced actin-interacting protein; KRAP; mKIAA1927; protein ITPRID2; SPA; SPAG13; sperm specific antigen 2; sperm-specific antigen 2 homolog; Ss; Ssfa2.
Gene ID
70599
Accession Number
NP_542125.3
Reactivity
Mouse, Mus musculus
Target
Sperm-specific antigen 2 homolog
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ITPRID2 ELISA Kit (Mouse) (OKEH08727) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ITPRID2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ITPRID2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ITPRID2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
78-5000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81% (n = 20)
Sensitivity
38pg/ml
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Protein ITPRID2
NCBI Gene Symbol
Itprid2
Protein Name
Q922B9
Gene Name URL
Itprid2
Nucleotide Accession Number
NM_080558.4

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