CKMT1B ELISA Kit (Human)

CAT: 0247-OKEH08700-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH08700-96WSize:96 Tests
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Creatine kinase, mitochondrial 1A, creatine kinase, mitochondrial 1B
Gene Aliases
Acidic-type mitochondrial creatine kinase; CKMT; CKMT1; creatine kinase U-type, mitochondrial; creatine kinase, mitochondrial 1 (ubiquitous) ; mia-CK; ubiquitous mitochondrial creatine kinase; U-MtCK; UMTCK.
Gene ID
1159, 548596
Accession Number
NP_001015001.1
Reactivity
Homo sapiens, Human
Target
Creatine kinase U-type, mitochondrial
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CKMT1B, CKMT1A ELISA Kit (Human) (OKEH08700) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CKMT1B, CKMT1A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CKMT1B, CKMT1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CKMT1B, CKMT1A captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
3.12-200U/L
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 117% (n = 20)
Sensitivity
1.54U/L
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Creatine kinase U-type, mitochondrial
NCBI Gene Symbol
CKMT1A, CKMT1B
Protein Name
P12532
Gene Name URL
CKMT1A, CKMT1B
Nucleotide Accession Number
NM_001015001.2

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