CKMT1A ELISA Kit (Human)

CAT: 0247-OKCD01504-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD01504-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Creatine Kinase, Mitochondrial 1A (CKMT1A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Creatine Kinase, Mitochondrial 1A (CKMT1A) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Creatine kinase, mitochondrial 1A|creatine kinase, mitochondrial 1B
Gene Aliases
Acidic-type mitochondrial creatine kinase; CKMT; CKMT1; creatine kinase U-type, mitochondrial; creatine kinase, mitochondrial 1 (ubiquitous) ; mia-CK; ubiquitous mitochondrial creatine kinase; U-MtCK; UMTCK.
Gene ID
1159|548596
Accession Number
NP_001015001.1
Reactivity
Homo sapiens|Human
Target
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) . Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Creatine Kinase, Mitochondrial 1A (CKMT1A) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Creatine Kinase, Mitochondrial 1A (CKMT1A) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Creatine Kinase, Mitochondrial 1A (CKMT1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Creatine Kinase, Mitochondrial 1A (CKMT1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
1.56-100ng/mL
Recovery
Matrix Recovery Range (%) Average (%) Serum (n=5) 90-97 93 EDTA Plasma (n=5) 90-101 96 Heparin Plasma (n=5) 99-105 102 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) Serum (n=5) 90-97 93 Serum (n=5) 90-97 93 EDTA Plasma (n=5) 90-101 96 EDTA Plasma (n=5) 90-101 96 Heparin Plasma (n=5) 99-105 102 Heparin Plasma (n=5) 99-105 102
Sensitivity
< 0.54 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-CKMT1A Microplate 96 Wells (12 x 8 Well Strips) CKMT1A Lyophilized Standard 2 x 100 ng 100X Biotinylated CKMT1A Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-CKMT1A Microplate 96 Wells (12 x 8 Well Strips) Anti-CKMT1A Microplate 96 Wells (12 x 8 Well Strips) CKMT1A Lyophilized Standard 2 x 100 ng CKMT1A Lyophilized Standard 2 x 100 ng 100X Biotinylated CKMT1A Detector Antibody 120 uL 100X Biotinylated CKMT1A Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBIJ4
Specificity
This assay has high sensitivity and excellent specificity for detection of Creatine Kinase, Mitochondrial 1A (CKMT1A) . No significant cross-reactivity or interference between Creatine Kinase, Mitochondrial 1A (CKMT1A) and analogues was observed.
Protein Length
417
NCBI Gene Symbol
CKMT1A|CKMT1B
Protein Name
Creatine kinase U-type, mitochondrial
Gene Name URL
CKMT1A|CKMT1B
Nucleotide Accession Number
NM_001015001.2

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