DEFA3 ELISA Kit (Mouse)

CAT: 0247-OKEH07651-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH07651-96WSize:96 Tests
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Defensin, alpha, 17, defensin, alpha, 3
Gene Aliases
Alpha-defensin 17; Alpha-defensin 3; AU014719; Cryp; Cryp17; crypt defensin 17; cryptdin 17; Defa27; Defa3; Defcr; Defcr-; Defcr1; Defcr17; Defcr3; Defcr-3; defensin, alpha, 27; defensin-related cryptdin-17; defensin-related cryptdin-3.
Gene ID
13237, 23855
Accession Number
NP_001161262.1
Reactivity
Mouse, Mus musculus
Target
Alpha-defensin 3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology DEFA3, DEFA17 ELISA Kit (Mouse) (OKEH07651) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DEFA3, DEFA17 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DEFA3, DEFA17 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DEFA3, DEFA17 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 117% (n = 20)
Sensitivity
0.22ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Alpha-defensin 3
NCBI Gene Symbol
Defa17, Defa3
Protein Name
P28310
Gene Name URL
Defa17, Defa3
Nucleotide Accession Number
NM_001167790.1

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