ERGIC1 ELISA Kit (Mouse)

Mean Inter-Assay Precision -

7732-18-5

Endoplasmic reticulum-golgi intermediate compartment (ERGIC) 1

1200007D18Rik; endoplasmic reticulum-Golgi intermediate compartment protein 1; ERGIC-32; ER-Golgi intermediate compartment 32 kDa protein; maa-136.

67458

NP_057543

Mouse, Mus musculus

Endoplasmic reticulum-Golgi intermediate compartment protein 1

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Aviva Systems Biology Ergic1 ELISA Kit (Mouse) (OKEH06527) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ergic1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ergic1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ergic1 captured in the well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, plasma and other biological fluids

78-5000pg/mL

Mean recovery when spiking into Serum and Plasma = 88.8%

41.9 pg/mL

Store as indicated in product manual.

Natural and recombinant Mouse Endoplasmic reticulum-Golgi intermediate compartment protein 1

Ergic1

Q9DC16

Ergic1