EMP1 ELISA Kit (Mouse)

Mean Inter-Assay Precision -

7732-18-5

Casein kappa

AW208918; Cs; CSN; CSN10; Csnk; kappa-casein.

12994

NP_001104759

Mouse, Mus musculus

Kappa-casein

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Aviva Systems Biology Emp1 ELISA Kit (Mouse) (OKEH06508) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Emp1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Emp1 and incubated. The Emp1 found in the sample or standards competes with the biotinylated Emp1 for limited binding sites on the immobilized anti-Emp1 antibody. Excess unbound biotinylated Emp1 and sample or standard Emp1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Emp1 captured in the well and inversely proportional to the amount of Emp1 which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, plasma and other biological fluids

0.78-50ng/mL

Mean recovery when spiking into Serum and Plasma = 105.4%

0.435 ng/mL

Store as indicated in product manual.

Natural and recombinant Mouse Kappa-casein

Csn3

P06796

Csn3