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IL2RB ELISA Kit (Mouse)

CAT: 0247-OKEH06430-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06430-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Interleukin 2 receptor, beta chain
Gene Aliases
CD122; high affinity IL-2 receptor subunit beta; IL-15 receptor beta chain; IL15R; IL-15R; IL15Rbeta; IL-15Rbeta; IL-2 receptor beta chain; IL-2 receptor subunit beta; IL-2/15 receptor-beta; Il-2/15Rbeta; Il-2R; IL-2R subunit beta; IL-2RB; Il-2Rbeta; interleukin-2 receptor subunit beta; p70; p70-75.
Gene ID
16185
Accession Number
NP_031976
Reactivity
Mouse, Mus musculus
Target
Interleukin-2 receptor subunit beta
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Il2rb ELISA Kit (Mouse) (OKEH06430) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Il2rb has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Il2rb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Il2rb captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 86%
Sensitivity
15.7 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Interleukin-2 receptor subunit beta
NCBI Gene Symbol
Il2rb
Protein Name
P16297
Gene Name URL
Il2rb
Nucleotide Accession Number
NM_007950

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