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ECH1 ELISA Kit (Rat)

CAT: 0247-OKEH06250-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH06250-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Enoyl-CoA hydratase 1
Gene Aliases
Delta (3,5) -Delta (2,4) -dienoyl-CoA isomerase, mitochondrial; enoyl CoA hydratase 1, peroxisomal; enoyl coenzyme A hydratase 1, peroxisomal; enoyl hydratase-like protein, peroxisomal; Peroxisomal enoyl hydratase-like protein; Pxel.
Gene ID
64526
Accession Number
NP_072116.1
Reactivity
Rat, Rattus norvegicus
Target
Delta (3,5) -Delta (2,4) -dienoyl-CoA isomerase, mitochondrial
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology Ech1 ELISA Kit (Rat) (OKEH06250) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ech1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ech1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ech1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.78-50ng/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 108%
Sensitivity
0.392 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Delta (3,5) -Delta (2,4) -dienoyl-CoA isomerase, mitochondrial
Protein Length
327
NCBI Gene Symbol
Ech1
Protein Name
Q62651
Gene Name URL
Ech1
Nucleotide Accession Number
NM_022594.1

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