ECH1 ELISA Kit (Human)

• Reproducibility :

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name :

  Enoyl-CoA hydratase 1

• Gene Aliases :

  Delta (3,5) -Delta (2,4) -dienoyl-CoA isomerase, mitochondrial; delta3,5-delta2,4-dienoyl-CoA isomerase; delta3,5-delta2,4-dienoyl-coenzyme A isomerase; dienoyl-CoA isomerase; enoyl Coenzyme A hydratase 1, peroxisomal; enoyl-CoA hydratase 1, peroxisomal; epididymis secretory sperm binding protein; HPXEL; peroxisomal enoyl-CoA hydratase 1.

• Gene ID :

  1891

• Accession Number :

  NP_001389

• Reactivity :

  Homo sapiens|Human

• Target :

  Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.

• Type :

  ELISA Kit

• Applications :

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method :

  Colorimetric

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type :

  Serum, plasma, tissue homogenates, cell lysates and other biological fluids.

• Detection Range :

  0.156-10ng/mL

• Recovery :

  Matrix Recovery Range (%) Average (%) serum (n=5) 90-104 94 EDTA plasma (n=5) 78-103 98 heparin plasma (n=5) 89-97 93 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 90-104 94 serum (n=5) 90-104 94 EDTA plasma (n=5) 78-103 98 EDTA plasma (n=5) 78-103 98 heparin plasma (n=5) 89-97 93 heparin plasma (n=5) 89-97 93

• Sensitivity :

  < 0.058 ng/mL

• Reconstitution :

  2°C to 8°C|-20°C

• Components :

  Component Amount Anti-ECH1 Microplate 96 Wells (12 x 8 Well Strips) ECH1 Lyophilized Standard 2 x 10 ng 100X Biotinylated ECH1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-ECH1 Microplate 96 Wells (12 x 8 Well Strips) Anti-ECH1 Microplate 96 Wells (12 x 8 Well Strips) ECH1 Lyophilized Standard 2 x 10 ng ECH1 Lyophilized Standard 2 x 10 ng 100X Biotinylated ECH1 Detector Antibody 120 uL 100X Biotinylated ECH1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• Notes :

  Formerly GWB-KBBCD3

• Specificity :

  This assay has high sensitivity and excellent specificity for detection of Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) . No significant cross-reactivity or interference between Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) and analogues was observed.

• Protein Length :

  328

• NCBI Gene Symbol :

  ECH1

• Protein Name :

  Delta (3,5) -Delta (2,4) -dienoyl-CoA isomerase, mitochondrial

• Gene Name URL :

  ECH1

• CAS Number :

  7732-18-5

• Nucleotide Accession Number :

  NM_001398.2