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PTGDS ELISA Kit (Mouse)

CAT: 0247-OKEH05912-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05912-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
_x005F Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Prostaglandin D2 synthase (brain)
Gene Aliases
21kDa; glutathione-independent PGD synthase; glutathione-independent PGD synthetase; lipocalin-type prostaglandin-D synthase; L-P; L-PGDS; PGD; PGD2; PGD2 synthase; PGDS; PGDS2; prostaglandin D2 synthase (21 kDa, brain) ; prostaglandin-D2 synthase; prostaglandin-H2 D-isomerase; Ptg; Ptgs3.
Gene ID
19215
Accession Number
NP_032989.2
Reactivity
Mouse, Mus musculus
Target
Prostaglandin-H2 D-isomerase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ptgds ELISA Kit (Mouse) (OKEH05912) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ptgds has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ptgds is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ptgds captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 89%
Sensitivity
0.399 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Prostaglandin-H2 D-isomerase
Protein Length
189
NCBI Gene Symbol
Ptgds
Protein Name
O09114
Gene Name URL
Ptgds
Nucleotide Accession Number
NM_008963.2

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