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HBB-BS ELISA Kit (Mouse)

CAT: 0247-OKEH05780-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05780-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Hemoglobin, beta adult major chain, hemoglobin, beta adult s chain, hemoglobin, beta adult t chain
Gene Aliases
AA409645; beta maj; beta major globin; beta s; beta t; beta1; Beta-1-globin; beta-globin; Beta-s; beta-single; Beta-t; Hbb1; Hbbt1; Hbbt2; Hemoglobin beta-1 chain; Hemoglobin beta-major chain; hemoglobin subunit beta-1; hemoglobin subunit beta-1-like; hemoglobin, beta adult s chain; hemoglobin, beta adult t chain; MommeD; MommeD7.
Gene ID
100503605, 101488143, 15129
Accession Number
NP_001188320.1
Reactivity
Mouse, Mus musculus
Target
Hemoglobin subunit beta-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology HBB-BS ELISA Kit (Mouse) (OKEH05780) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HBB-BS has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for HBB-BS is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HBB-BS captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 104%
Sensitivity
0.448 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Hemoglobin subunit beta-1
Protein Length
147
NCBI Gene Symbol
Hbb-b1, Hbb-bs, Hbb-bt
Protein Name
P02088
Gene Name URL
Hbb-b1, Hbb-bs, Hbb-bt
Nucleotide Accession Number
NM_001201391.1