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HBB ELISA Kit (Rat)

CAT: 0247-OKEH03193-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03193-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Hemoglobin subunit beta
Gene Aliases
Beta-1-globin; hemoglobin beta chain complex; hemoglobin beta chain, major-form; hemoglobin beta-1 chain; hemoglobin subunit beta-1; hemoglobin, beta; III beta-1 globin; III beta-2 globin.
Gene ID
24440
Accession Number
NP_150237.1
Reactivity
Rat, Rattus norvegicus
Target
Hemoglobin subunit beta-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology HBB ELISA Kit (Rat) (OKEH03193) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hbb has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Hbb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hbb captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.78-50ng/mL
Sensitivity
0.449 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
16 kDa
Specificity
Natural and recombinant Rat Hemoglobin subunit beta-1
Protein Length
147
NCBI Gene Symbol
Hbb
Protein Name
P02091
Gene Name URL
Hbb
Nucleotide Accession Number
NM_033234.1