ERCC5 ELISA Kit (Mouse)

CAT: 0247-OKEH05695-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH05695-96WSize:96 Well
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
DNA repair protein complementing XP-G cells homolog
Gene Aliases
DNA excision repair protein ERCC-5; Xeroderma pigmentosum group G-complementing protein homolog.
Gene ID
22592
Accession Number
NP_035859.2
Reactivity
Mouse, Mus musculus
Target
DNA repair protein complementing XP-G cells homolog
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ercc5 ELISA Kit (Mouse) (OKEH05695) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ercc5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ercc5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ercc5 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 89%
Sensitivity
0.09 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse DNA repair protein complementing XP-G cells homolog
Protein Length
1170
NCBI Gene Symbol
ERCC5
Protein Name
P35689
Gene Name URL
ERCC5
Nucleotide Accession Number
NM_011729.2

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