ERCC5 ELISA Kit (Human)

CAT: 0247-OKCD07749-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD07749-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Xeroderma Pigmentosum, Complementation Group G (XPG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Xeroderma Pigmentosum, Complementation Group G (XPG) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
ERCC excision repair 5, endonuclease
Gene Aliases
COFS3; DNA excision repair protein ERCC-5; DNA repair protein complementing XP-G cells; ERCC5-201; ERCM2; excision repair cross-complementation group 5; excision repair cross-complementing rodent repair deficiency, complementation group 5; UVDR; Xeroderma pigmentosum group G-complementing protein; xeroderma pigmentosum, complementation group G; XPG; XPGC; XPG-complementing protein.
Gene ID
2073
Accession Number
NP_000114.2
Reactivity
Homo sapiens|Human
Target
Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER) . Acts as a cofactor for a DNA glycosylase that removes oxidized pyrimidines from DNA. May also be involved in transcription-coupled repair of this kind of damage, in transcription by RNA polymerase II, and perhaps in other processes too.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Xeroderma Pigmentosum, Complementation Group G (XPG) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Xeroderma Pigmentosum, Complementation Group G (XPG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Xeroderma Pigmentosum, Complementation Group G (XPG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Xeroderma Pigmentosum, Complementation Group G (XPG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
< 0.128ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount ERCC5 Microplate 96 Wells (12 x 8 Well strips) ERCC5 Lyophilized Standard 2 100X ERCC5 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount ERCC5 Microplate 96 Wells (12 x 8 Well strips) ERCC5 Microplate 96 Wells (12 x 8 Well strips) ERCC5 Lyophilized Standard 2 ERCC5 Lyophilized Standard 2 100X ERCC5 HRP-Detector Anitbody 1 x 120 uL 100X ERCC5 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
NCBI Gene Symbol
ERCC5
Protein Name
DNA repair protein complementing XP-G cells
Gene Name URL
ERCC5
Nucleotide Accession Number
NM_000123.3

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