Products for Research Use Only

MAPRE1 ELISA Kit (Mouse)

CAT: 0247-OKEH05222-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKEH05222-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Microtubule-associated protein, RP/EB family, member 1
Gene Aliases
5530600P05Rik; adenomatosis polyposis coli binding protein Eb1; AI462499; AI504412; APC-binding protein EB1; AW260097; BIM; BIM1p; D2Ertd459; D2Ertd459e; Eb1; end-binding protein 1; microtubule-associated protein RP/EB family member 1.
Gene ID
13589
Accession Number
NP_031922.1
Reactivity
Mouse, Mus musculus
Target
Microtubule-associated protein RP/EB family member 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Mapre1 ELISA Kit (Mouse) (OKEH05222) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mapre1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mapre1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mapre1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 89.4%
Sensitivity
0.09 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Microtubule-associated protein RP/EB family member 1
Protein Length
268
NCBI Gene Symbol
Mapre1
Protein Name
Q61166
Gene Name URL
Mapre1
Nucleotide Accession Number
NM_007896.3