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MTPAP ELISA Kit (Mouse)

CAT: 0247-OKEH05050-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH05050-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Mitochondrial poly (A) polymerase
Gene Aliases
0610027A18Rik; AW551379; Pa; PAP associated domain containing 1; PAP-associated domain-containing protein 1; Papd1; poly (A) RNA polymerase, mitochondrial; polynucleotide adenylyltransferase; Tent; Tent6.
Gene ID
67440
Accession Number
NP_080433.1
Reactivity
Mouse, Mus musculus
Target
Poly (A) RNA polymerase, mitochondrial
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Mtpap ELISA Kit (Mouse) (OKEH05050) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mtpap has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mtpap is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mtpap captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 90.7%
Sensitivity
41.4 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Poly (A) RNA polymerase, mitochondrial
Protein Length
585
NCBI Gene Symbol
Mtpap
Protein Name
Q9D0D3
Gene Name URL
Mtpap
Nucleotide Accession Number
NM_026157.2

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