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MTPAP ELISA Kit (Human)

CAT: 0247-OKEH01808-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH01808-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Mitochondrial poly (A) polymerase
Gene Aliases
MtPAP; PAP-associated domain-containing protein 1; PAPD1; poly (A) RNA polymerase, mitochondrial; polynucleotide adenylyltransferase; SPAX4; TENT6; terminal uridylyltransferase 1; TUTase 1.
Gene ID
55149
Accession Number
NP_060579.3
Reactivity
Homo sapiens, Human
Target
Poly (A) RNA polymerase, mitochondrial
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology MTPAP ELISA Kit (Human) (OKEH01808) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human MTPAP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated MTPAP and incubated. The MTPAP found in the sample or standards competes with the biotinylated MTPAP for limited binding sites on the immobilized anti-human MTPAP antibody. Excess unbound biotinylated MTPAP and sample or standard MTPAP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated MTPAP captured in the well and inversely proportional to the amount of MTPAP which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
Sensitivity
0.13 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
66 kDa
Specificity
Natural and recombinant Human Poly (A) RNA polymerase, mitochondrial
Protein Length
582
NCBI Gene Symbol
MTPAP
Protein Name
Q9NVV4
Gene Name URL
MTPAP
Nucleotide Accession Number
NM_018109.3

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