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Gp49a ELISA Kit (Mouse)

CAT: 0247-OKEH04703-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04703-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Leukocyte immunoglobulin-like receptor, subfamily B, member 4B
Gene Aliases
Glycoprotein 49 A; gp4; gp49; Gp49a; Lilr4b; mast cell surface glycoprotein Gp49A.
Gene ID
14727
Accession Number
NP_032173.1
Reactivity
Mouse, Mus musculus
Target
Mast cell surface glycoprotein Gp49A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Gp49a ELISA Kit (Mouse) (OKEH04703) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gp49a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Gp49a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gp49a captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
1.56-100ng/ml
Sensitivity
0.814 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
34 kDa
Specificity
Natural and recombinant Mouse Mast cell surface glycoprotein Gp49A
Protein Length
303
NCBI Gene Symbol
Lilrb4b
Protein Name
Q61450
Gene Name URL
Lilrb4b
Nucleotide Accession Number
NM_008147.2

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