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Gdf15 ELISA Kit (Mouse)

CAT: 0247-OKEH04596-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04596-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Growth differentiation factor 15
Gene Aliases
Growth/differentiation factor 15; macrophage inhibiting cytokine-1; macrophage inhibitory cytokine 1; MIC-; MIC-1; NAG; NAG-1; SBF.
Gene ID
23886
Accession Number
NP_035949.2
Reactivity
Mouse, Mus musculus
Target
Growth/differentiation factor 15
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Gdf15 ELISA Kit (Mouse) (OKEH04596) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gdf15 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Gdf15 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gdf15 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids
Detection Range
31.2-2000pg/mL
Sensitivity
15.6 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
33 KDa
Specificity
Natural and recombinant Mouse Growth/differentiation factor 15
Protein Length
303
NCBI Gene Symbol
Gdf15
Protein Name
Q9Z0J7
Gene Name URL
Gdf15
Nucleotide Accession Number
NM_011819.2

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