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GDF15 ELISA Kit (Human)

CAT: 0247-OKEH02756-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH02756-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Growth differentiation factor 15
Gene Aliases
GDF-15; growth/differentiation factor 15; macrophage inhibitory cytokine 1; MIC1; MIC-1; NAG-1; non-steroidal anti-inflammatory drug-activated gene-1; NRG-1; NSAID (nonsteroidal anti-inflammatory drug) -activated protein 1; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; PDF; PLAB; placental bone morphogenetic protein; placental TGF-beta; prostate differentiation factor; PTGFB; PTGF-beta.
Gene ID
9518
Accession Number
NP_004855.2
Reactivity
Homo sapiens, Human
Target
Growth/differentiation factor 15
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology GDF15 ELISA Kit (Human) (OKEH02756) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GDF15 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GDF15 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GDF15 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
Sensitivity
7.8 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
34 kDa
Specificity
Natural and recombinant Human Growth/differentiation factor 15
Protein Length
308
NCBI Gene Symbol
GDF15
Protein Name
Q99988
Gene Name URL
GDF15
Nucleotide Accession Number
NM_004864.2

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