GDF15 ELISA Kit (Rat)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 247 440 26 269 409 Standard deviation 1.31 10.12 18.92 1.37 13.18 22.9 CV (%) 4.7 4.1 4.3 5.3 4.9 5.6 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 28 247 440 26 269 409 Mean (pg/ml) 28 247 440 26 269 409 Standard deviation 1.31 10.12 18.92 1.37 13.18 22.9 Standard deviation 1.31 10.12 18.92 1.37 13.18 22.9 CV (%) 4.7 4.1 4.3 5.3 4.9 5.6 CV (%) 4.7 4.1 4.3 5.3 4.9 5.6

• Gene Name:

  Growth differentiation factor 15

• Gene Aliases:

  Gdf 15, GDF15, GDF-15, MIC 1, MIC1, MIC-1, NAG-1, NSAID-activated gene 1 protein, NSAID-regulated gene 1 protein, PDF, PLAB, PTGFB

• Gene ID:

  29455

• Accession Number:

  NP_062089.1

• Reactivity:

  Rat

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: S189-A303

• Target:

  GDF-15 (Growth differentiation factor 15), also known as TGF-PL, MIC-1, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by directly interfering with chemokine signaling and integrin activation.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's rat GDF-15 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GDF-15 has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: S189-A303) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDF-15 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat GDF-15 amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)

• Detection Range:

  15.6 pg/ml - 1,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity:

  Natural and recombinant rat GDF-15

• NCBI Gene Symbol:

  GDF15

• Protein Name:

  Growth/differentiation factor 15

• Gene Name URL:

  GDF15

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_019216.2