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Ace ELISA Kit (Mouse)

CAT: 0247-OKEH04499-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04499-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
Gene Aliases
Angiotensin-converting enzyme; AW208573; CD143; dipeptidyl carboxypeptidase I; dipeptidyl peptidase; kininase II.
Gene ID
11421
Accession Number
NP_033728.1
Reactivity
Mouse, Mus musculus
Target
Angiotensin-converting enzyme
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ace ELISA Kit (Mouse) (OKEH04499) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ace has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ace is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ace captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
39 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
151 KDa
Specificity
Natural and recombinant Mouse Angiotensin-converting enzyme
Protein Length
1312
NCBI Gene Symbol
Ace
Protein Name
P09470
Gene Name URL
Ace
Nucleotide Accession Number
NM_009598.2