ACE Chemi-Luminescent ELISA Kit (Mouse)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Angiotensin I Converting Enzyme (ACE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Angiotensin I Converting Enzyme (ACE) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Angiotensin I converting enzyme (peptidyl-dipeptidase A) 1

• Gene Aliases:

  Angiotensin-converting enzyme; AW208573; CD143; dipeptidyl carboxypeptidase I; dipeptidyl peptidase; kininase II.

• Gene ID:

  11421

• Accession Number:

  NP_033728

• Reactivity:

  Mouse|Mus musculus

• Target:

  Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm.

• Type:

  ELISA Kit

• Applications:

  Chemiluminescent immunoassay

• Detection Method:

  Chemiluminescent

• Assay Principle:

  The microplate provided in this kit has been pre-coated with an antibody specific to Angiotensin I Converting Enzyme (ACE) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Angiotensin I Converting Enzyme (ACE) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Angiotensin I Converting Enzyme (ACE) level in the sample or standard.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma, tissue homogenates, cerebrospinal fluid, cell lysates, cell culture supernates and other biological fluids

• Detection Range:

  54.9-40,000pg/mL

• Recovery:

  Matrix Recovery range (%) Average (%) Serum (n=5) 98-105 101 EDTA Plasma (n=5) 95-105 102 Heparin Plasma (n=5) 98-105 101 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 98-105 101 Serum (n=5) 98-105 101 EDTA Plasma (n=5) 95-105 102 EDTA Plasma (n=5) 95-105 102 Heparin Plasma (n=5) 98-105 101 Heparin Plasma (n=5) 98-105 101

• Sensitivity:

  54.9 pg/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Ace Microplate 96 Wells (12 x 8 Well strips) Ace Lyophilized Standard 2 100X Ace Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount Ace Microplate 96 Wells (12 x 8 Well strips) Ace Microplate 96 Wells (12 x 8 Well strips) Ace Lyophilized Standard 2 Ace Lyophilized Standard 2 100X Ace Biotinylated Detector Anitbody 1 x 120 uL 100X Ace Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL

• NCBI Gene Symbol:

  Ace

• Protein Name:

  Angiotensin-converting enzyme

• Gene Name URL:

  Ace

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_009598