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NAA20 ELISA Kit (Mouse)

CAT: 0247-OKEH04327-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04327-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
N (alpha) -acetyltransferase 20, NatB catalytic subunit
Gene Aliases
1500004D14Rik;2900026I01Rik; AU041458; D2Ertd186; D2Ertd186e; methionine N-acetyltransferase; Na; N-acetyltransferase 5; N-acetyltransferase 5 (ARD1 homolog, S. cerevisiae) ; N-alpha-acetyltransferase 20; Nat5; natB catalytic subunit; natB complex subunit NAT5; N-terminal acetyltransferase B complex catalytic subunit NAA20; N-terminal acetyltransferase B complex catalytic subunit NAT5.
Gene ID
67877
Accession Number
NP_001135437.1
Reactivity
Mouse, Mus musculus
Target
N-alpha-acetyltransferase 20
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Naa20 ELISA Kit (Mouse) (OKEH04327) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Naa20 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Naa20 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Naa20 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
78-5000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
39.2 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
20 kDa
Specificity
Natural and recombinant Mouse N-alpha-acetyltransferase 20
Protein Length
178
NCBI Gene Symbol
Naa20
Protein Name
P61600
Gene Name URL
Naa20
Nucleotide Accession Number
NM_001141965.1

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