IGSF1 ELISA Kit (Mouse)

CAT: 0247-OKEH04182-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH04182-96WSize:96 Well
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Immunoglobulin superfamily, member 1
Gene Aliases
5330413N23;5530402E03; AI747649; betaglycan and inhibin binding protein; immunoglobulin superfamily member 1; inhBP; InhBP/p120; inhibin-binding protein; mKIAA0364.
Gene ID
209268
Accession Number
NP_808259.2
Reactivity
Mouse, Mus musculus
Target
Immunoglobulin superfamily member 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Igsf1 ELISA Kit (Mouse) (OKEH04182) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Igsf1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Igsf1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Igsf1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.156-10ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
Sensitivity
0.086 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
147 kDa
Specificity
Natural and recombinant Mouse Immunoglobulin superfamily member 1
Protein Length
1317
NCBI Gene Symbol
Igsf1
Protein Name
Q7TQA1
Gene Name URL
Igsf1
Nucleotide Accession Number
NM_177591.4

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