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WISP1 ELISA Kit (Rat)

CAT: 0247-OKEH04082-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04082-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Cellular communication network factor 4
Gene Aliases
CCN family member 4; ELM-1; Wisp1; WISP-1; WNT1 inducible signaling pathway protein 1.
Gene ID
65154
Accession Number
NP_113904.1
Reactivity
Rat, Rattus norvegicus
Target
WNT1-inducible-signaling pathway protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Wisp1 ELISA Kit (Rat) (OKEH04082) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Wisp1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Wisp1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Wisp1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/ml
Sensitivity
7.88 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
41 kDa
Specificity
Natural and recombinant Rat WNT1-inducible-signaling pathway protein 1
Protein Length
367
NCBI Gene Symbol
Ccn4
Protein Name
Q99PP0
Gene Name URL
Ccn4
Nucleotide Accession Number
NM_031716.1

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