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WISP1/CCN4 ELISA Kit (Human)

CAT: 0247-OKBB00538-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00538-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 236 1087 1826 352 1240 1910 SD 7.55 46.7 89.5 16.2 64.5 128 CV% 3.2 4.3 4.9 4.6 5.2 6.7 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 236 1087 1826 352 1240 1910 Mean (pg/mL) 236 1087 1826 352 1240 1910 SD 7.55 46.7 89.5 16.2 64.5 128 SD 7.55 46.7 89.5 16.2 64.5 128 CV% 3.2 4.3 4.9 4.6 5.2 6.7 CV% 3.2 4.3 4.9 4.6 5.2 6.7
CAS Number
7732-18-5
Gene Name
Cellular communication network factor 4
Gene Aliases
CCN family member 4, CCN4, WISP1, WISP1c, WISP1i, WISP1tc, Wnt 1 inducible signaling pathway protein 1, Wnt-1 induced secreted protein, WNT1 induced secreted protein 1, WNT1 inducible signaling pathway protein 1, WNT1 inducible-signaling pathway protein 1
Gene ID
8840
Accession Number
NP_001191798.1
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: T23-N367
Target
WISP1 (WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed. By use of radiation hybrid mapping panels, the WISP1 was mapped to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. It found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84% of tumors examined compared with patient-matched normal mucosa.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human WISP1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for WISP1 has been precoated onto 96-well plates. Standards (NS0, T23-N367) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for WISP1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human WISP1 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernatants, serum and plasma (heparin, EDTA)
Detection Range
31.2 pg/ml - 2,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human WISP1
NCBI Gene Symbol
CCN4
Protein Name
CCN family member 4
Gene Name URL
CCN4
Nucleotide Accession Number
NM_001204869.1

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