WISP1 Chemi-Luminescent ELISA Kit (Human)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level WNT1 Inducible Signaling Pathway Protein 1 (WISP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level WNT1 Inducible Signaling Pathway Protein 1 (WISP1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Cellular communication network factor 4

• Gene Aliases:

  CCN family member 4; WISP1; WISP1 3'UTR-associated RNA 1; WISP1 overlapping transcript 1 (non-protein coding) ; WISP1c; WISP1i; WISP1-OT1; WISP1tc; WISP1-UT1; WNT1 induced secreted protein 1; WNT1 inducible signaling pathway protein 1; Wnt-1-induced secreted protein.

• Gene ID:

  8840

• Accession Number:

  NP_001191798

• Reactivity:

  Homo sapiens|Human

• Target:

  Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X (L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan.

• Type:

  ELISA Kit

• Applications:

  Chemiluminescent immunoassay

• Detection Method:

  Chemiluminescent

• Assay Principle:

  The microplate provided in this kit has been pre-coated with an antibody specific to WNT1 Inducible Signaling Pathway Protein 1 (WISP1) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to WNT1 Inducible Signaling Pathway Protein 1 (WISP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the WNT1 Inducible Signaling Pathway Protein 1 (WISP1) level in the sample or standard.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma, tissue homogenates and other biological fluids

• Detection Range:

  2.74-2,000pg/mL

• Recovery:

  Matrix Recovery range (%) Average (%) Serum (n=5) 98-105 101 EDTA Plasma (n=5) 88-105 102 Heparin Plasma (n=5) 94-102 98 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 98-105 101 Serum (n=5) 98-105 101 EDTA Plasma (n=5) 88-105 102 EDTA Plasma (n=5) 88-105 102 Heparin Plasma (n=5) 94-102 98 Heparin Plasma (n=5) 94-102 98

• Sensitivity:

  31.2 pg/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount WISP1 Microplate 96 Wells (12 x 8 Well strips) WISP1 Lyophilized Standard 2 100X WISP1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount WISP1 Microplate 96 Wells (12 x 8 Well strips) WISP1 Microplate 96 Wells (12 x 8 Well strips) WISP1 Lyophilized Standard 2 WISP1 Lyophilized Standard 2 100X WISP1 Biotinylated Detector Anitbody 1 x 120 uL 100X WISP1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL

• NCBI Gene Symbol:

  CCN4

• Protein Name:

  WNT1-inducible-signaling pathway protein 1

• Gene Name URL:

  CCN4

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001204869