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NOS3 ELISA Kit (Dog)

CAT: 0247-OKEH03970-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03970-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Nitric oxide synthase 3 (endothelial cell)
Gene Aliases
Constitutive NOS; EC-NOS; Endothelial NOS; NOS type III.
Gene ID
403784
Accession Number
NP_001003158.2
Reactivity
Canis lupus, Dog
Target
Nitric oxide synthase, endothelial
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology NOS3 ELISA Kit (Dog) (OKEH03970) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NOS3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NOS3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NOS3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
3.12-200U/mL
Sensitivity
1.37U/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
1205 kDa
Specificity
Natural and recombinant Dog Nitric oxide synthase, endothelial
Protein Length
1205
NCBI Gene Symbol
NOS3
Protein Name
Q9TUX8
Gene Name URL
NOS3
Nucleotide Accession Number
NM_001003158.2

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