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NOS3 ELISA Kit (Rat)

CAT: 0247-OKEH02990-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH02990-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Nitric oxide synthase 3
Gene Aliases
CNOS; constitutive NOS; EC-NOS; endothelial nitric oxide synthase 3; endothelial NOS; eNos; nitric oxide synthase 3, endothelial cell; nitric oxide synthase, endothelial; NOS type III; NOSIII.
Gene ID
24600
Accession Number
NP_068610.1
Reactivity
Rat, Rattus norvegicus
Target
Nitric oxide synthase, endothelial
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology NOS3 ELISA Kit (Rat) (OKEH02990) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-rat Nos3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Nos3 and incubated. The Nos3 found in the sample or standards competes with the biotinylated Nos3 for limited binding sites on the immobilized anti-rat Nos3 antibody. Excess unbound biotinylated Nos3 and sample or standard Nos3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Nos3 captured in the well and inversely proportional to the amount of Nos3 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/ml
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
133 kDa
Specificity
Natural and recombinant Rat Nitric oxide synthase, endothelial
Protein Length
1202
NCBI Gene Symbol
Nos3
Protein Name
Q62600
Gene Name URL
Nos3
Nucleotide Accession Number
NM_021838.2

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