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LIPE ELISA Kit (Pig)

CAT: 0247-OKEH03917-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03917-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Lipase E, hormone sensitive type
Gene Aliases
Hormone-sensitive lipase; HSL; monoacylglycerol lipase LIPE; REH; retinyl ester hydrolase.
Gene ID
397583
Accession Number
NP_999480.1
Reactivity
Porcine, Sus scrofa
Target
Hormone-sensitive lipase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LIPE ELISA Kit (Pig) (OKEH03917) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LIPE has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LIPE is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LIPE captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.094 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
764 kDa
Specificity
Natural and recombinant Pig Hormone-sensitive lipase
Protein Length
764
NCBI Gene Symbol
LIPE
Protein Name
Q68J42
Gene Name URL
LIPE
Nucleotide Accession Number
NM_214315.1

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