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LIPE ELISA Kit (Human)

CAT: 0247-OKCD02692-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02692-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Lipase, Hormone Sensitive (LIPE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Lipase, Hormone Sensitive (LIPE) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Lipase E, hormone sensitive type
Gene Aliases
AOMS4; FPLD6; hormone-sensitive lipase; HSL; LHS; lipase, hormone-sensitive; monoacylglycerol lipase LIPE; REH; retinyl ester hydrolase.
Gene ID
3991
Accession Number
NP_005348.2
Reactivity
Homo sapiens|Human
Target
In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Hormone Sensitive (LIPE) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase, Hormone Sensitive (LIPE) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase, Hormone Sensitive (LIPE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lipase, Hormone Sensitive (LIPE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Matrix Recovery range (%) Average (%) _x005F Serum (n=5) 89-104 98 _x005F EDTA Plasma (n=5) 92-101 97 _x005F Heparin Plasma (n=5) 84-105 95 _x005F Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 89-104 98 Serum (n=5) 89-104 98 EDTA Plasma (n=5) 92-101 97 EDTA Plasma (n=5) 92-101 97 Heparin Plasma (n=5) 84-105 95 Heparin Plasma (n=5) 84-105 95
Sensitivity
0.065 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount _x005F Anti-LIPE Microplate 96 Wells (12 x 8 Well strips) _x005F LIPE Lyophilized Standard 2 _x005F 100X Biotinylated LIPE Detector Antibody 1 x 120 uL _x005F 100X Avidin-HRP Conjugate 1 x 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F Stop Solution 1 x 6 mL _x005F TMB Substrate 1 x 9 mL _x005F Component Amount Component Amount Anti-LIPE Microplate 96 Wells (12 x 8 Well strips) Anti-LIPE Microplate 96 Wells (12 x 8 Well strips) LIPE Lyophilized Standard 2 LIPE Lyophilized Standard 2 100X Biotinylated LIPE Detector Antibody 1 x 120 uL 100X Biotinylated LIPE Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Lipase, Hormone Sensitive (LIPE) ._x005F No significant cross-reactivity or interference between Lipase, Hormone Sensitive (LIPE) and analogues was observed.
Protein Length
1076
NCBI Gene Symbol
LIPE
Protein Name
Hormone-sensitive lipase
Gene Name URL
LIPE
Nucleotide Accession Number
NM_005357.3

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