LIPE ELISA Kit (Mouse)

CAT: 0247-OKEH03330-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH03330-96WSize:96 Well
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Lipase, hormone sensitive
Gene Aliases
4933403G17Rik; H; hormone-sensitive lipase; HSL; monoacylglycerol lipase LIPE; REH; retinyl ester hydrolase.
Gene ID
16890
Accession Number
NP_001034596.1
Reactivity
Mouse, Mus musculus
Target
Hormone-sensitive lipase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LIPE ELISA Kit (Mouse) (OKEH03330) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lipe has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Lipe is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lipe captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
1.56-100U/L
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
Sensitivity
0.78U/L
Reconstitution
Store as indicated in product manual.
Molecular Weight
83 kDa
Specificity
Natural and recombinant Mouse Hormone-sensitive lipase
Protein Length
759
NCBI Gene Symbol
Lipe
Protein Name
P54310
Gene Name URL
Lipe
Nucleotide Accession Number
NM_001039507.2

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