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WFIKKN2 ELISA Kit (Mouse)

CAT: 0247-OKEH03678-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03678-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2
Gene Aliases
2610304F08Rik; AY100450; Ga; Gasp1; GASP-1; growth and differentiation factor-associated serum protein 1; mGASP-1; WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2; WFIKKNRP.
Gene ID
278507
Accession Number
NP_861540.2
Reactivity
Mouse, Mus musculus
Target
WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology WFIKKN2 ELISA Kit (Mouse) (OKEH03678) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Wfikkn2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Wfikkn2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Wfikkn2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
Sensitivity
40.3 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
63 kDa
Specificity
Natural and recombinant Mouse WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2
Protein Length
571
NCBI Gene Symbol
Wfikkn2
Protein Name
Q7TQN3
Gene Name URL
Wfikkn2
Nucleotide Accession Number
NM_181819.2

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