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WFIKKN2 ELISA Kit (Human)

CAT: 0247-OKCA01591-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA01591-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2
Gene Aliases
GASP-1, WFDC20B, hGASP-1, WFIKKNRP
Gene ID
124857
Accession Number
NP_001317270.1
Reactivity
Human
Target
Protease-inhibitor that contains multiple distinct protease inhibitor domains. Probably has serine protease- and metalloprotease-inhibitor activity. Inhibits the biological activity of mature myostatin, but not activin.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology WFIKKN2 ELISA Kit (Human) (OKCA01591) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for WFIKKN2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for WFIKKN2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample WFIKKN2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.156 - 10 ng/mL
Recovery
Sample Mean Recovery (%) Range (%) Serum (n=5) 84 80-89 EDTA Plasma (n=4) 95 91-100 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 84 80-89 Serum (n=5) 84 80-89 EDTA Plasma (n=4) 95 91-100 EDTA Plasma (n=4) 95 91-100
Sensitivity
0.039 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
WFIKKN2
Protein Name
WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2
Gene Name URL
WFIKKN2
Nucleotide Accession Number
NM_001330341.1

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